Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with FHIT Recombinant Rabbit Monoclonal Antibody (104) (Product # MA5-29276, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).